Fig. 3.
Casein agar assay for SpeB proteolytic activity. MGAS5005, Δsrv and MGAS5005Δsrv(pIAβ8-srv) were stab inoculated into agar plates containing skim milk and incubated for 18 h microaerophilically. Protease activity is manifest as a zone of translucence surrounding the stab sites. There is a marked increase in the amount of SpeB proteolysis exhibited by Δsrv compared to MGAS5005 or MGAS5005Δsrv(pIAβ8-srv).