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Detection of SpeB in GAS biofilms. Western immunoblot analysis failed to detect the presence of SpeB in supernatants collected from MGAS5005 and MGAS5005ΔspeB after growth for 24 h in a biofilm state. Elevated levels of SpeB were detected in supernatants collected from MGAS5005Δsrv. Low levels of the inactive form of SpeB (zymogen) were detected in supernatants collected from the srv-complemented strain MGAS5005Δsrv(pIAβ8-srv).
