Table 1.
MALDI-TOF, matrix assisted laser desorption ionization-Time of flight mass spectrometry.
Eleven protein spots selected were individually analyzed. The spot number (Spot No.) corresponds to the position marked on the gel (Fig. 1). Identified proteins and accession numbers were derived from the MS-Fit search algorithm.
*MW (kDa) represents molecular size of protein.
†pI is an isoelectric point, where the net charge is zero.
‡D means the value obtained from the database.
§E indicates the value estimated from spots on the SDS gel.
∥Sequence coverage (%) is defined as the ratio of the portion of peptide sequence covered by the matched protein to the whole length of peptide sequence of the spot.