Figure 1. CD8⁺ and NK1.1⁺ γδ T cell subsets undergo homeostatic expansion in TCRβ^−/−δ^−/− mice at distinct rates.

(A and B) Splenic γδ T cells were isolated from TCRβ^−/− mice, labeled with CFSE, and injected into TCRβ^−/−/δ^−/− recipients. Homeostatic proliferation of CD8⁻ and CD8⁺ or NK1.1⁻ and NK1.1⁺ γδ T cells subsets was assessed on day 5 by flow cytometry. Gates delineate those cells that have undergone at least one division, as determined by the CFSE levels of non-T cells within our donor population (unfilled grey histogram). (C) The frequency of NK1.1⁻/CD8⁻, NK1.1⁻/CD8⁺, and NK1.1⁺/CD8⁻ in the donor γδ T cell population was determined by flow cytometry. Percentages of live CD3⁺/TCRδ⁺ cells are shown for each quadrant. (D) γδ T cell reconstitution was assessed from 3 days to 2 months after transfer into TCRβ^−/−/δ^−/− mice.