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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: J Immunol. 2009 Feb 15;182(4):1892–1900. doi: 10.4049/jimmunol.0803165

Figure 3. TCR variable gene expression by γδ T cell subsets.

Figure 3

Splenic γδ T cells were isolated from TCRβ−/− mice and analyzed by flow cytometry for Vγ and Vδ expression before (A and B) or after adoptive transfer into TCRβ−/−/δ−/− recipients (C and D). γδ T cell subsets were gated based on CD8α (A) or NK1.1 (B) expression, and the percentage of cells that express Vγ4, Vδ4, Vδ5, Vδ6.3, Vδ6λ12, or Vδ8 was determined. CD8+ (C) and CD8− (D) γδ T cell subsets were also characterized for Vγ and Vδ expression after undergoing homeostatic expansion in TCRβ−/−/δ−/− mice for two months. (E–G) TCRβ−/− splenic γδ T cells were analyzed by flow cytometry for expression of Vγ1, Vγ4, and CD8α either before (E and G) or after (F and G) adoptive transfer into TCRβ−/−/δ−/− recipients. (E) Percentage of live CD3+/TCRδ+ cells is shown. (ns = not statistically significant; ** P < 0.01)