Figure 7. Blocking NKG2A or Ly49C interactions with MHCI does not affect γδ T cell homeostatic expansion.
Splenic γδ T cells were enriched from TCRβ−/− mice and injected into TCRβ−/−/δ−/− recipients. Recipient mice received injections of control IgG, anti-NKG2, or anti-Ly49C/I antibodies on days 0, 2, 5, 8, and 11 relative to cell transfer. Reconstitution of total γδ T cells (A) and NK1.1+ γδ T cells (B), or total number of NK cells (C, NK1.1+/TCRδ−) was determined by flow cytometry on day 14. (D) NKG2A and Ly49CIFH expression by NK cells was determined by flow cytometry at the time of harvest. (ns = not statistically significant)