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. 2009 Jul 14;3(7):e480. doi: 10.1371/journal.pntd.0000480

Figure 3. Impaired capacity of antigen-specific CD4+ T cells to express cytokines.

Figure 3

Groups of BALB/c and CBA mice (n = 8) were infected with L. major parasites for two (A and B) or four (C) weeks. Individual popliteal lymph nodes and footpads (pool from at least two footpads) were harvested and the % of cytokine-expressing CD4+ T cells was determined by flow cytometry. (3A) dot plot profiles of cytokine-expressing CD4+ T cells isolated from the popliteal lymph nodes and the footpads two weeks post infection; (3B) % of cytokine-expressing CD4+ T cells two weeks post infection. (3C) % of cytokine-expressing CD4+ T cells four weeks post infection. Error bars represent standard deviations. Isotype control for IFN-γ: 0.23±0.05%, IL-4: 0.31±0.08% and IL-10: 0.26±0.06%. All cytokines were below the detection limit when the cells were stimulated in the absence of PMA/ionomycin stimulation. The detection limits were defined as % isotype control +3 standard deviations: IFN-γ = 0.38%, IL-4 = 0.55% and IL-10 = 0.44%. Data show the results of one representative experiment out of three independent experiments.