Skip to main content
. 2009 Jul 9;4(7):e6207. doi: 10.1371/journal.pone.0006207

Figure 5. Injection of rIL-6 in IL-6KO mice decreased chronic disease severity following CB3/LPS treatment.

Figure 5

(A) Representative Masson's Trichrome stained cardiac section from C57BL/6 or IL-6KO mice. Mice were treated with CB3/LPS and either DMEM or rIL-6 on days 0, 1, 2 and 3 post infection. Mice developed disease pathology as determined by fibrosis in blue and immune cell infiltration within the fibrosis areas by 28 days PI (C57BL/6 with DMEM n = 15, IL-6KO with DMEM n = 11, IL-6KO with rIL-6 n = 9). Magnification:400×. (B) Chronic cardiac disease histology was scored blindly by a four tier grading system to determine severity differences: grade 1, 0–10% pathology; grade 2, 11–25%; grade 3, 26–50%; grade 4, greater than 50% (black circles indicate wt mice with DMEM, white circles indicate IL-6KO mice with DMEM, grey circles indicate IL-6KO mice with rIL-6) (bar is mean±SE, *p<0.05). Disease severity was found to be decreased in the IL-6KO mice treated with rIL-6 compared to DMEM treated IL-6KO mice.