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. 2000 Sep 5;97(19):10418–10423. doi: 10.1073/pnas.190318397

Figure 3.

Figure 3

The NES in helix 4 of the coiled-coil domain of Stat1 leads to nuclear exclusion of a fusion protein. The fluorescence micrographs of transiently transfected 293T cells expressing GFP linked to helix 4 (a, b), helix 1 (c, d), or GFP alone (e, f) reveal the different subcellular distribution of chimeric constructs of low molecular weight. Only the fusion protein linked to helix 4 is excluded from the nucleus (a). The localization of GFP and the GFP fusions are depicted in a, c, and e, and the corresponding positions of nuclei stained with Hoechst 33258 are documented in b, d, and f.