Figure 2.

Northern blot analysis of NGF-promoted gene regulation in (A) PC12 cells and (B) PC12-cell-nnr5 derived cells with (T14) and without (nnr5) Trk expression. Total cellular RNA was isolated from cells treated without (naïve) or with NGF for 7–14 days (NGF). Ten micrograms of RNA was analyzed as described in Materials and Methods with the indicated probes. Blots were stripped and reprobed with β-actin to indicate relative loading. Ratios to side of blots indicate numbers of tags in PC12 cell SAGE analysis +/− NGF.