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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2009 May 3;47(3):269–274. doi: 10.1016/j.freeradbiomed.2009.04.027

Figure 3. Activation of sGC in SH-SY5Y cells and HPASMC and GSH levels in HPASMC.

Figure 3

A. Cells were incubated with various concentrations of CysNO in HBSS for 60 minutes. IBMX (1mM) was present during incubation of HPASMC to inhibit phosphodiesterases. cGMP levels were measured with an EIA kit. Results represent the amount of cGMP produced by cells in a single well of a 6-well plate. The data represent mean ± SEM (n=3). B. HPASMC were treated with various concentrations of CysNO for 60 minutes in HBSS. GSH levels in cell lysates were determined by HPLC. Data were normalized to protein concentration and represent mean ± SEM (n=3).