FIG. 6.

Effect of 25OHD and 1,25(OH)₂D on bacterial infection and infection-associated cell death in trophoblastic cells. A) The 3A cells were pretreated with 25OHD (100 nM), 1,25(OH)₂D (10 nM), or vehicle (0.1% ethanol) for 48 h and then infected with E. coli for 24 h. After removal of exogenous bacteria, monolayers of 3A cells were lysed and assessed for surviving intracellular E. coli (cfu). B) The 3A cells were pretreated with 25OHD (1–100 nM), 1,25(OH)₂D (0.1–10 nM), or vehicle (0.1% ethanol) for 48 h and then infected with E. coli for 24 h. After removal of exogenous bacteria, cell death was assessed by analysis of the release of LDH into cell culture supernatants. Data are shown as the level of LDH in supernatants from infected 3A cells relative to infected vehicle-treated cells (C). *Statistically different from vehicle samples, P < 0.05; **statistically different from vehicle samples, P < 0.01; ***statistically different from vehicle samples, P < 0.001.