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Rab7aT22N blocks VEGFR2 trafficking. A-C, Endothelial cells expressing GFP-Rab7aT22N (green) were stimulated with VEGF-A for 0 (A), 30 (B) or 120 (C) min and labeled for VEGFR2 (red) and nuclei (blue). Bar, 10 μm. D, Quantification of VEGFR2-labeled cells overexpressing GFP-Rab7aT22N after VEGF-A stimulation (*, p<0.05).
