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. Author manuscript; available in PMC: 2009 Jun 30.
Published in final edited form as: Neurobiol Dis. 2008 Feb 15;30(2):221–233. doi: 10.1016/j.nbd.2008.01.009

Fig. 7.

Fig. 7

Co-expression of Cx32 mutants with Cx31.3. These are images of HeLa cells that express Cx31.3 and the various Cx32 mutants as indicated. The cells are labeled with a rabbit antiserum against Cx31.3 (726; red) and a mouse monoclonal antibody against Cx32 (green), and counterstained with DAPI; merged images are shown in the right column. Note that all cells appear to express Cx31.3, which is found on the cell membrane (arrows) and diffusely within the cytoplasm, but not in GJ plaques. Not all cells express Cx32. Some Cx32 mutants show diffuse intracellular staining (A, B, F), and others are found in intracellular puncta (C, D, E); only wild type Cx32 (G) forms GJ plaques (arrowheads). Cx31.3-immunoreactivity in double expressing cells is similar to the cells that express only Cx31.3; none of the Cx32 mutants appear to alter the localization of Cx31.3. Scale bar: 10 μm.