Table 1.

Determination of CD4 T cell diversity in a healthy control and two patients.

Subject (time-point) [CD4 T cells per μl blood*]	No. of Vβ17+ CD4+ T cells analyzed	Rearrangement	Frequency of Vβ17 gene (%)	Frequency of Jβ (1.4, 1.5 or 2.6) gene (%)	Peak size (%)	No. of distinct sequences within the peak(s)	Diversity (×106)
Healthy control	105	Vβ17-Jβ1.5	1.6	13	18.5	140	0.4
	106	Vβ17-Jβ1.4	1.6	2.9	20.2	60	0.6
	105	Vβ17-Jβ1.4	1.6	2.9	20.2	60	0.6
SSc patient (pretransplant)	106	Vβ17-Jβ1.4	1.0	0.6	35.1	12	0.7
	105	Vβ17-Jβ1.4	1.0	0.6	35.1	11	0.6
SSc patient (1 mo posttransplant) [CD4 = 26]	105	Vβ17-Jβ1.4	0.7	6.7	100.0	12	0.3
	105	Vβ17-Jβ2.6	0.7	2.0	100.0	25	0.2
SSc patient (12 mo posttransplant) [CD4 = 347]	105	Vβ17-Jβ1.4	1.5	2.1	100.0	16	0.5
	105	Vβ17-Jβ2.6	1.5	1.1	100.0	70	0.4
MS patient (1 mo posttransplant) [CD4 = 5]	105	Vβ17-Jβ1.4	0.5	3.3	100.0	74	0.4
	105	Vβ17-Jβ2.6	0.5	1.7	100.0	45	0.5
MS patient (12 mo posttransplant) [CD4 = 186]	105	Vβ17-Jβ1.4	0.5	2.8	100.0	230	1.6
	105	Vβ17-Jβ2.6	0.5	1.8	100.0	>112**	>1.2

^*5^th-95^th percentile reference range, 416-1437/μl.

^**Plateau of the curve showing the number of distinct clones vs the number of clones sequenced had not been reached by the time 180 clones were sequenced.