FIGURE 7.

Synthesis and dicing of dsRNA do not require a specific RDRC composition in vitro. (A) Products of dsRNA synthesis untreated or treated with Nuclease S1 prior to electrophoresis. Assays were performed using a 79-nt ssRNA template and radiolabeled CTP. The intensity of reaction product correlates with the level of Rdr1 present in the preparation (not shown). (B) Small RNA products of coupled dsRNA synthesis and dicing assays using the same 79-nt ssRNA template as in A and RDRC complexes that were gently washed to preserve Dcr2 association. The intensity of reaction product correlates with the level of Dcr2 protein present in the preparation (not shown).