FIGURE 1.

An ATPase-deficient UPF1 mutant, UPF2, and UPF3b localize to P-bodies in HeLa cells. (A) HeLa cells were stained with patient autoimmune serum IC6, which mainly contains α-Ge-1 antibodies, to visualize the P-bodies (shown in red; left column). HA-tagged UPF1 WT or the ATPase-deficient HA-UPF1 mut1 (Kashima et al. 2006) was expressed from transfected plasmids and detected with α-HA antibody (shown in yellow; middle column). The merge of both channels is shown in the right column. Endogenous (B) UPF2 and (C) UPF3b was visualized using rabbit polyclonal antisera against the respective proteins (Lykke-Andersen et al. 2000). Insets in the upper-left corner of the pictures in panels B and C show a single P-body zoomed in.