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. 2009 Jul;15(7):1274–1281. doi: 10.1261/rna.1423309

FIGURE 2.

FIGURE 2.

Tel-sRNA generation is correlated with genome reorganization and heterochromatin formation in Tetrahymena thermophila. (A) Northern blot analyses of RNAs collected at different times of conjugation (shown on top) from wild-type cells. For RNase treatment, RNAs extracted at 4 h of conjugation were used. (B) Quantitative RT-PCR of tel-sRNAs using RNA samples in A. Signals were presented as fold differences relative to Tel-C signal in 0 h of conjugation, which was arbitrarily set as 1. (C) Northern blot analyses of RNAs collected at different time of conjugation (shown on top) from or Dcl1(−/−) cells. In both A and C, the blots were hybridized with (ACCCCA)3, (TGGGGT)3, or M-scnRNA probes, respectively. The transcripts were indicated on the right. Ethidium bromide stained PAGE gel was shown at bottom as the loading control.