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. 2009 Jul 1;20(13):3003–3011. doi: 10.1091/mbc.E09-03-0186

Figure 4.

Figure 4.

She1p affects the cell cycle–dependent recruitment of Jnm1p (dynamitin) and Ldb18p (p24) to astral MTs. (A and B) Colocalization of Jnm1-3GFP and mCherry-Tub1 in (A) wild-type and (B) she1Δ G1, pre-anaphase, or anaphase cells, as indicated. (C and D) Colocalization of Ldb18-3GFP and mCherry-Tub1 in (C) wild-type and (D) she1Δ G1, pre-anaphase, or anaphase cells, as indicated. Jnm1-3GFP and Ldb18-3GFP foci primarily localize to SPBs in G1 and pre-anaphase cells and then appear on aMTs during anaphase. White arrowheads denote Jnm1-3GFP or Ldb18-3GFP localized at an astral MT plus end. Loss of She1p permits enhanced association of Jnm1-3GFP and Ldb18-3GFP with aMTs throughout the cell cycle. Scale bars, 5 μm. (E and F) The percentage of cells with Jnm1-3GFP (E) or Ldb18-3GFP (F) foci localized to aMTs or SPBs as a function of cell cycle stage. (G and H) Percentage of cells with Jnm1-3GFP (G) or Ldb18-3GFP (H) present on astral MTs (n >50). Error bars represent SD.