Table 1.
Mutation | Name | Plasmid no. |
---|---|---|
E23A | E23A | pRP1732 |
E26A | E256A | pRP1733 |
D27A | D27A | pRP1734 |
E23A, E25A, D27A | EED triple | pRP1736 |
49 SKLDf53 to 49AAAAA53 | Loop A | pRP1737 |
87TVTDES92 to 87AAAAAA92 | Loop B | pRP1738 |
Q148AE149A | QE | pRP1739 |
174KKK176 to 174AAA176 | Loop C | pRP1740 |
184FDEKT188 to 184AAAAA188 | Helix swap | pRP1741 |
213PGF215 to 213AAA215 | PGF triple | pRP1744 |
P213A | P213A | pRP1742 |
G214A | G214A | pRP1743 |
Loop A + loop B | Loop A+B | pRP1771 |
Loop B + loop C | Loop B+C | pRP1772 |
Loop A + loop C | Loop A+C | pRP1773 |