Colocalization of ILK with ELMO2 and RhoG. (A) Primary mouse keratinocytes were cotransfected with the indicated fluorescent proteins. Forty-eight hours after transfection, the cells were briefly trypsinized and replated onto a laminin 332 matrix. Keratinocytes were allowed to adhere and spread, and after 2 h they were processed for confocal microscopy. (B) Primary mouse keratinocytes were cotransfected with plasmids encoding mCherry-tagged ILK and the indicated GFP-tagged ELMO2 proteins. Forty-eight hours after transfection, the cells were briefly trypsinized and replated onto a laminin 332 matrix. Keratinocytes were allowed to adhere and spread, and after 2 h they were processed for confocal microscopy. Insets represent magnification of boxed areas. (C) Primary mouse keratinocytes were cotransfected with mCherry-tagged ILK and the indicated GFP-tagged RhoG proteins. Forty-eight hours after transfection, the cells were trypsinized and replated onto a laminin 332 matrix. Keratinocytes were allowed to adhere and spread, and after 2 h they were processed for confocal microscopy. Insets represent magnification of boxed areas. Bar, 20 μm.