Figure 2.
Antibodies to C_850038 recognize the IC97 subunit of I1 dynein. (A) The IC97 antibody was used to probe Western blots of axonemes derived from wild-type and several dynein mutants. The antibody recognizes a band, with an Mr of ∼90,000 that is present in wild type (WT) and dynein mutants that are defective in the outer dynein arm (oda2), or inner arm subtypes a, c, and d (ida4). This band is specifically missing in I1-dynein mutants (ida1, ida3, and ida7) that fail to assemble I1 dynein in the axoneme. The lower panel contains the Coomassie-stained gel showing protein loads. (B) Western blots of FPLC fractions from oda2 dynein extracts were probed with the IC97 antibody (top). The band recognized by the antibody cofractionates with the I1-dynein complex in the dynein-f peak (detected with antibodies to the IC138 subunit; bottom) confirming that the antibody specifically recognizes the IC97 I1-dynein subunit (Porter et al., 1992; Pazour et al., 1998; Yang and Sale, 1998; Kamiya et al., 1991; King and Dutcher, 1997; Myster et al., 1997; Harrison et al., 1998; Bowman et al., 1999; Pazour and Witman, 2000; Perrone et al., 1998, 2000; DiBella et al., 2004b,c; Hendrickson et al., 2004).