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. 2009 Jul 1;20(13):3044–3054. doi: 10.1091/mbc.E09-04-0276

Figure 4.

Figure 4.

IC97 is not required for I1-dynein assembly or anchoring in the axoneme. (A) Western blots of partial I1-dynein assembly mutants bop5-1, ida7-1::IC140 5A, and bop5-2. IC97 assembles into axonemes of both ida7-1::IC140 5A and bop5-1 but does not localize to the axoneme when IC138, FAP120, and LC7b are missing (bop5-2). Bottom, Coomassie-stained gel of the same samples. (B) FPLC fractions of dynein extracts from a double mutant ida7-1::IC140 5A oda9 demonstrate that IC97, while assembled in the axoneme, does not cofractionate with I1 dynein, but rather is detected in earlier fractions. (C) Western blots of axonemes from a new LC8 mutant, fla14-3 that expresses a larger LC8 protein (top, left; Yang, Yang, Wirschell, and Davis, unpublished data), demonstrates that IC97 and FAP120 fail to assemble in the axoneme (bottom). The rest of the I1-dynein complex seems to assemble in the absence of IC97/FAP120 (represented by IC140; top, right).