Table 2.
Chlamydomonas strains used in this study
| Strain | Defect | Motility | References |
|---|---|---|---|
| CC124 | WT | Harris (1989, 2009) | |
| CC1331 (fla14-3)a,b | Radial spokes, I1 dynein, retrograde IFT | Paralyzed | Yang et al., unpublished |
| CC-4077 (ida7-1::IC140 5A)c | Truncated IC140 | WT | Perrone et al. (1998) |
| ida7-1::IC140 5A oda9 | Truncated IC140; lacks outer dynein arm | Slow-jerky swimming | This study |
| CC-4080 (bop5-1) | Partial I1 dynein assembly; IC138 point mutant | Slow-smooth swimming | Dutcher et al. (1988); Hendrickson et al. (2004) |
| bop5-2 | Partial I1 dynein assembly; null IC138 allele | Very slow swimming, spinning cells | Bower et al. (2009); Dutcher et al. (1988) |
| CC-2664 (ida1) | Lacks I1 dynein; 1αHC mutant | Slow-smooth swimming | Kamiya et al. (1991); Myster et al. (1997) |
| CC-2666 (ida2) | Lacks I1 dynein; 1βHC mutant | Slow-smooth swimming | Kamiya et al. (1991); Perrone et al. (2000) |
| CC-2668 (ida3) | Lacks I1 dynein; gene product unknown. | Slow-smooth swimming | Kamiya et al. (1991) |
| CC-2670 (ida4) | Lacks inner arm isoforms a, c, and d | Slow swimming | Kamiya et al. (1991) |
| CC-3921 (ida7) | Lacks I1 dynein; IC140 mutant | Slow-smooth swimming | Perrone et al. (1998); Yang and Sale (1998) |
| CC-262 (pf17 mt-) | Lacks radial spoke head; RSP1 mutant | Paralyzed | McVittie (1972); Huang et al. (1981) |
| 5c12B | HA-tagged α1-tubulin | WT | Kozminski et al. (1993) |
| pf28 pf30 ssh1d | Lacks outer dynein arm; Lacks I1 dynein; suppressor of short mutation | Paralyzed | Ledizet and Piperno (1986); Freshour et al. (2007) |
a Yang, Yang, Wirschell, and Davis (unpublished) determined that the pf5 mutation in strain CC1331 is an allele at the FLA14 locus (fla114-3) that encodes the LC8 protein. Other strains denoted as pf5 in the Chlamydomonas Stock Center contain a mutation that is tightly linked to the FLA14 locus and thus are not fla14-3/pf5 alleles.
b Assembly defects in fla14-3 include an effect on the retrograde IFT motor as evidenced by assembly of half to full-length flagella; no defects in assembly of the outer dynein arm are observed; I1-dynein assembly defects are restricted to loss of IC97 and FAP120 specifically; and the radial spokes are reduced resulting in the paralyzed flagellar phenotype.
c The ida7-1::IC140 5A strain was first described in Perrone et al. (1998).
d Strain pf28 pf30 ssh1 is a triple mutant lacking both the outer dynein arm and I1 dynein; the ssh1 mutation allows for wild-type length flagella in the double dynein mutant background (LeDizet and Piperno, 1995; Freshour et al., 2006).