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. 2009 Jun 19;7:49. doi: 10.1186/1479-5876-7-49

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Copyright © 2009 Pulaski et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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VLC express CD52. A. RT-PCR demonstrating CD52 mRNA expression in VLCs FACS isolated from 4 ovarian tumors (NTC-no template control). B. qRT-PCR quantification of CD52 mRNA expression in FACS-isolated VLC and tumor endothelial cells (TECs). C. FACS analysis confirming CD52 protein expression on CD45⁺/VE-Cadherin⁺VLC. VLC do not express the T cell marker CD4. CD45^(-)/VE-Cadherin⁺tumor endothelial cells do not express CD52. D. Immunofluorescence demonstrating co-expression of VE-Cadherin (red) and anti-CD52 (green) in ovarian cancer. Arrows indicate CD52⁺/VE-Cadherin^(-)lymphocytes.