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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Stroke. 2009 May 28;40(7):2546–2551. doi: 10.1161/STROKEAHA.109.547265

Figure 3. PRV labeling of pyramidal neurons in the bilateral cerebral cortices at the bregma level.

Figure 3

PRV-614-mRFP (red) was injected into the left forelimb muscles. Single layer confocal images on coronal brain sections at the bregma level show that in a normal mouse, the pyramidal neurons were labeled with PRV primarily in the corresponding area of the right cortex (A) and sparsely in the left cortex (B). PRV-positive pyramidal cells in the right cortex were reduced 11 days after MCAo (C), while the number PRV-labeled cells was little increased in the contralesional cortex (D). In contrast, the PRV-positive cells were increased 32 days after stroke in both ipsilesional (E) and contralesional hemispheres (F) compared with 11 days. Inserts in A and B, schematic drawing of the brain coronal sections showing the position of the photomicrograph appearing in the left and right panels, respectively. Scale bar=100 μm.