Table 1.
Sequences and dissociation constants of selected aptamers. The enriched aptamer pool was cloned into E. coli and fifty colonies were randomly picked for sequencing. Sequence alignment revealed 6 consensus sequence groups, and one representative sequence from each group was synthesized and its affinity was measured via fluorescence. All sequences exhibited high affinity for streptavidin and their Kd ranged from 25 to 65 nM.
| Clone Name | Sequence | Dissociation Constant (Kd) |
|---|---|---|
| 10 | GTGATCGTCCAGCGACCGAGCAGGAAACTTATGTAACGACCCGAAATTCCTGCTTAGACT | 25 nM |
| 02 | ATTTGCAACACTTACCACTAAAAGGGCCGACGACCGATGATGTTAGGTCCAGTGCTTTCT | 35 nM |
| 50 | CAAGAACTCCTAAGTATAATGTGAGGGATCCGAAATTCTGCTCTTATGTATGGCAAGATT | 35 nM |
| 07 | ATGGCTGTGGTGGAGGGCCGAAGCCTGTACAAAACAGGAATGCGCTTATCTTGGAGTAT | 50 nM |
| 05 | CAGCGGGCATTTCTAGGGCCATCTTTAATCTTCTTTATGGAGAGCCTCGTTCACACGTTG | 60 nM |
| 24 | TCGAATAGCCCCCCGCGTCCGTCTTCCTTTAGCGATCTATGCTCCTTTGTATGGTCGGGC | 65 nM |