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. 2009 Apr 7;30(7):1082–1088. doi: 10.1093/carcin/bgp078

Fig. 2.

Fig. 2.

Delay of hollow lumen formation in 3D culture. (A) Acini formed by RWPE-1/rA1 cells showed delayed luminal clearing compared with parental RWPE-1 or RWPE-1/NC cells. Micrographs were generated of acinar structures visualized by phase-contrast microscopy (top) or of structures that were fixed, stained with 4′,6-diamidino-2-phenylindole to stain nuclein acids and rhodamine-phalloidin to label filamentous actin, and visualized by confocal fluorescence microscopy (bottom). Bar, 50 μm. (B) Acini with complete or incomplete hollow lumens from NC or rA1 cells were enumerated and presented as a mean percentage ± SD of total counted, **P < 0.01. (C) The diameters of acini were measured and represented as percentage of total counted; at least 100 acini were counted for each cell line. The color version of this figure is available at Carcinogenesis Online.