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. 2009 Apr 7;30(7):1082–1088. doi: 10.1093/carcin/bgp078

Fig. 4.

Fig. 4.

Autocrine IL-6 secretion induces phosphorylation of STAT3. (A) Conditioned medium (CM) was collected from RWPE-1/NC and rA1 cells cultured in basal medium for 24 h (NC CM and rA1 CM, respectively). Fresh cells (NC or rA1 as indicated on top) were washed with phosphate-buffered saline and incubated for 6 h in fresh K-SFM basal medium or conditioned medium from NC or rA1 cells. Mouse IgG as control or mouse anti-IL-6 was added at 1 μg/ml to block IL-6 activity in the indicated samples. Medium and additional IgG added to each sample are listed on left and indicated by ‘+’. Lysates were prepared and analyzed for phosphorylation of STAT3 (Y705) (pSTAT3) by immunoblotting. A representative experiment of three independent experiments is shown. (B) Relative band intensities of pSTAT3 in RWPE-1/NC cells were determined in relation to cells treated with basal medium for each of the three experiments. Bars represent the mean ± SD.