Table 2.
Kinetic properties of control and Δ23REC myofibrils at 10℃ during phosphate and ADP jumps
| Control | Δ23REC | |
|---|---|---|
Jump up: 1 to 3 mM Pi,  (s−1) |
74 ± 12 (n = 5) | 58 ± 6 (n = 5) |
Jump down: 3 to 1 mM Pi,  (s−1) |
3.8 ± 0.8 (n = 8) | 3.5 ± 0.2 (n = 9) |
| Tension at 1 mM Pi, P1 (mN mm−2) | 222 ± 17 (n = 10) | 47 ± 4 (n = 10) * |
| Tension at 3 mM Pi, P3 (mN mm−2) | 154 ± 13 (n = 10) | 30 ± 3 (n = 10) * |
| Rate of tension redevelopment at 1 mM Pi, ktr1 (s−1) | 7.1 ± 0.4 (n = 10) | 4.3 ± 0.5 (n = 10) * |
| Rate of tension re-development at 3 mM Pi, ktr2 (s−1) | 9.7 ± 0.7 (n = 9) | 7.0 ± 0.4 (n = 8) * |
| Jump up: 0 to 3 mM ADP, k+ADP (s−1) | 4.7 ± 0.6 (n = 8) | 3.6 ± 0.2 (n = 7) |
| Jump down: 3 to 0 mM ADP, k-ADP (s-1) | 4.2 ± 0.4 (n = 8) | 4.0 ± 0.5 (n = 7) |
| Tension at 3 mM ADP, Pd (mN mm−2) | 283 ± 20 (n = 8) | 53 ± 10 (n = 8)* |
| Rate of tension redevelopment at 3 mM ADP, ktrd (s−1) | 4.2 ± 0.2 (n = 8) | 1.9 ± 0.2 (n = 6)* |
Values are mean averages ± SEM * Statistically significantly different to the control (P < 0.05, Student’s t test)