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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Nat Neurosci. 2008 Dec 21;12(1):60–69. doi: 10.1038/nn.2238

Figure 4.

Figure 4

Neuronal PrP expression rescues the cookie finding phenotype. (a). Trial 1 for all lines, neuronal PrPC expressers in black (“+neuronal PrPC”; B6129, Tg20, NSE-PrP); neuronal PrPC-deficient mice in red (“−neuronal PrPC”; Zürich I PrP−/−, MBP-PrP, CD19-PrP, and Prn−/−). +neuronal PrPC minimum=19 s; lower quartile=53 s; median=70.5 s; upper quartile=133 s; maximum=569 s. −neuronal PrPC min=37 s; lower quartile=106.5 s; median=187 s; upper quartile=485.5 s; max=600 s. (b) Trial 2. Note reduced timescale of 5 min. +neuronal PrPC minimum=6 s; lower quartile=19.5 s; median=30 s; upper quartile=49 s; maximum=300 s. −neuronal PrPC min=23 s; lower quartile=64 s; median=140 s; upper quartile=286.5 s; max=300 s. In a,b individuals that failed the trial were given the conservative score of the total trial length. *** p<0.001, two-tailed Mann-Whitney test. (c-d) Breakdown by strain of data in a and b respectively. Filled dots represent strains expressing PrPC in neurons; open dots those that do not. Because it expressed PrPC only in some neurons, Lck-PrP (grey) was not included in either group in a,b. Lines represent medians. (e-f) Close-up for B6129, ZI PrP−/− and NSE-PrP, our 3 representative strains. (g) Altered phenotype of ZI PrP−/− mice in the habituation-dishabituation test. All mice habituate to the first odor (PB). B6129 (black) and NSE-PrP (grey) mice showed strong renewed interest in the novel odors (PB+vanilla mix and amyl acetate) while ZI PrP−/− mice (open dots) failed to respond to them. Error bars ±SEM * p<0.05, ** p<0.01, 2-way ANOVA, Bonferroni post test.