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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Nat Neurosci. 2008 Dec 21;12(1):60–69. doi: 10.1038/nn.2238

Figure 5.

Figure 5

Lck-PrP transgenic mice express some neuronal PrPC. Fluorescent in situ hybridization for detection of Prnp transcripts in the olfactory bulb and cerebellum of transgenic Lck-PrP mice (a-c) and CD19-PrP mice (d). Left panels show signal from antisense Prnp probe; right panels show negative control sense probes. The Prnp probe used here was such that it only recognized wild type Prnp transcripts, and not the truncated Prnp transcript that is produced from the Zürich I Prnp knockout allele. All slides were detected over equal amount of time. (a) Prnp is expressed in cells of the olfactory bulb (OB) in Lck-PrP mice. From left to right, arrows point to examples of a Prnp-positive cell in the external plexiform layer, a mitral cell, and granule cells. (b) Prnp expression in the cerebellum of Lck-PrP mice. Arrow points to an example of a Prnp-positive cell in the molecular layer. Some Purkinje cells and granule cells are also labeled. (c) Lck-PrP mice do not express Prnp in the olfactory epithelium (OE). (d) Prnp is not expressed in the OB of CD19-PrP mice. The low signal that can be observed is due to background staining. All scalebars 100 μm.