Figure 6.

Ah −/− mice have a high incidence of persistent hyaloid artery. To provide histological sections defining the extended hyaloid artery, eyes of Ah +/+ (A) and Ah −/− (B) were fixed in 10% buffered formalin and 6-μm sections were stained with Alcian Blue PAS. The lens (L), vitreous (V), retina (R), and hyaloid artery (HA) are shown. Eyes of Ah +/+ and Ah −/− mice were examined by indirect and direct ophthalmoscopy for hyaloid structures; a graph of hyaloid artery persistence is shown (C). The difference between Ah +/+ and Ah −/− mice was significant (P < 0.005), and the percentage of mice with extensive hyaloid artery is shown with the 95% upper confidence internal. Results were statistically evaluated by using the two-tailed Fisher's exact test (25).