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. 2009 Jul 1;23(13):1510–1521. doi: 10.1101/gad.1790709

Figure 5.

Figure 5.

The significance of extra density by additional density difference map analysis. (A) 3D reconstructions of c-Jun-IID (left), Sp1-IID (right), and 3D density difference map (center) of c-Jun-IID minus Sp1-IID are shown with the major lobes and channels as previously indicated in Figures 2–4. The yellow and green mesh represents positive and negative differences, respectively, with the gray mesh corresponding to the 3D structure of c-Jun-IID (shown at center). The orange circle denotes the significant positive difference on the surface of TFIID and is predicted as the position of c-Jun contact within TFIID. (B) Summed 3D density difference maps of each activator for p53-IID (top center), Sp1-IID (bottom left), and c-Jun-IID (bottom right). The summed positive density differences are shown in magenta (p53), red (Sp1), and yellow (c-Jun) and confirm the points of contact for each respective activator on TFIID. The green mesh represents the sum of the negative differences. Bar, 100 Å.