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Suppressor screen for the lethal phenotype of MPS2 deletion. (A) Spore analysis of MPS2/MPS2 and mps2Δ/MPS2 cells. a, b, c, and d indicate cells that developed from spores of one complete tetrad. The cells marked by an arrow did not contain the MPS2 gene. (B) SMY2 is a multicopy suppressor of the lethality of mps2Δ cells. mps2Δ pRS316-MPS2 cells were transformed with the indicated plasmids. Transformants were grown on SC-Leu or 5-FOA plates for 3 d at 23°C.
