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. 2009 Jul 1;23(13):1559–1570. doi: 10.1101/gad.524209

Figure 4.

Figure 4.

POM34 mRNA binds to SESA components. (A,B) The indicated yeast cells were tested for growth on SC-Ura and 5-FOA plates at 23°C. All strains harbored initially the pRS316-MPS2 plasmid. (C) Coimmunoprecipitation of POM34 mRNA together with Eap1, Scp160, and Asc1 by Smy2. Extracts from yeast cells were incubated with anti-HA-coated magnetic beads, with or without RNaseA treatment, and analyzed by immunoblotting with the indicated antibodies. RNA was isolated from the immunoprecipitates and analyzed by RT–PCR using primers specific to POM34, POM152, and NDC1. In this immunoprecipitation experiment the efficiency of Asc1 coimmunoprecipitation was reduced probably because of the longer incubation time due to RNaseA treatment (cf. Figs. 2 and 4C). Abbreviations are as in Figure 2B.