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. 2009 Jul 1;23(13):1559–1570. doi: 10.1101/gad.524209

Figure 7.

Figure 7.

Genetic interactions between SESA and POM34 with genes involved in SPB duplication. (A) Deletion of POM34 suppresses ndc1-1, bbp1-1, and mps2-42. The listed yeast cells were tested for growth on YPAD plates at indicated temperatures. (B) SMY2 is a multicopy suppressor of the cold-sensitive phenotype of ndc1-1. ndc1-1 cells transformed with pRS315-NDC1 or pRS425-SMY2 were tested for growth on YPAD plates at 14°C and 30°C. (C) Pom34 protein level is reduced in ndc1-1 2μm-SMY2 cells. Total cell extracts from yeast strains expressing POM34-6HA were analyzed by immunoblotting using anti-HA antibodies. Anti-Tub2 antibodies were used as loading control. The graph shows the quantification of three independent experiments, normalized for the wild-type protein levels. Bars are standard deviations around the mean value. (D) mps2Δ 2μm-SMY2 and mps2Δ pom34Δ cells are temperature sensitive for growth. The listed yeast cells were tested for growth on YPAD plates at indicated temperatures. (E) Deletion of SESA components enhances bbp1-1 growth defects. The listed yeast cells were tested for growth on YPAD plates at the indicated temperatures.