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Stereological cell counts in the substantia nigra pars compacta stained with tyrosine hydroxylase (TH) show fewer TH-positive neurons in aged GFRα-1+/− mice (30% cell loss; *P < 0.038). Although the number of TH-positive neurons in the ventral tegmental area was reduced (24%) in aged GFRα-1+/− mice as compared to aged wild-type mice, the difference was not significant.
