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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Cancer Biol Ther. 2006 Nov 30;5(11):1556–1562. doi: 10.4161/cbt.5.11.3461

Figure 2.

Figure 2

The minimal element to block cell migration is xkmviswxx. (A) PC3N cells were grown to a monolayer on laminin 322 and cell migration was induced by scratching the monolayer surface. The cells were incubated with the indicated alanine substitution sequences of HYD1 at a concentration of 50 μM in the presence of 1% FBS for 12 hours following the scratch. (B) PC3N cells were grown to a monolayer on laminin 322 and cell migration was induced by scratching the monolayer surface. The cells were incubated with the indicated deletion forms of the HYD1 sequences at a concentration of 50 μM in the presence of 1% FBS for 12 hours following the scratch. In both (A and B), the data represent the percent of cells in a microscopic field migrating into the scratch. Three fields were analyzed per sample and the error bars represent one standard deviation about the mean. The data obtained from both the alanine substitution and deletion analysis is collated in (C).