Skip to main content
. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Pain. 2007 Aug 15;132(1-2):154–168. doi: 10.1016/j.pain.2007.06.026

Fig. 7.

Fig. 7

(a) Femur sections were stained with tartrate-resistant acid phosphatase (TRAP) on day 10 to visualize osteoclasts that stained dark red (indicated by arrows). Osteoclast staining was increased in sarcoma-treated mice compared to control mice. (b) Sarcoma increased the number of osteoclasts within the metaphysis of the femur. Morphine infusion further increased osteoclasts compared to saline-infused mice. Osteoclast counts did not differ between morphine- and saline-infused mice treated with control media. (c) IL-1β protein content was measured in exudate from the intramedullary space of the femurs on day 12. Sustained morphine infusion increased IL-1β protein content in sarcoma-treated mice. Sustained morphine infusion had no effect on IL-1β protein levels in control media-treated mice. Graphs show means ± SEM. *Indicates significant difference from control saline group, #indicates significant difference between saline- and morphine-treated mice within the sarcoma or the control groups.