Figure 3.

Immunoprecipitation of bovine heart nucleids. Heart mitochondrial nucleoids were immunoprecipitated with antibodies as indicated (lanes 1–4). Analysis of the immunoprecipitates by Western blotting against SOD2 showed that the SOD2 is included in the nucleoid complex. SOD2 and GPx1 are constituents of a putative nucleoid antioxidant system. Nonspecific binding and cross-reactivity were excluded by appropriate controls (lanes 5 and 6; unspecific IgG and without antibody). Data shown are representative of 3 independent experiments.