Figure 5.

Estimation of freshly synthesized collagen by hepatocytes embedded in collagen gel between d 4 and 5 of culture. Control, proline, and 75% refer to monoculture of hepatocytes exposed continuously to control medium, proline (20 μg/ml)-supplemented control medium, and endothelial cell-conditioned medium, respectively. Cocultures of hepatocytes and endothelial cells were continuously treated with control medium. Under certain conditions, control or conditioned medium was supplemented with cis-hydroxyproline (40 μg/ml). *P < 0.05 vs. control; #P < 0.05 vs. cis-hydroxyproline supplemented cultures.