Figure 5.

Tissue homogenates from bat species show relatively low 20S proteasome chymotrypsin-like activity. A) 20S proteasome-specific activity in liver homogenates from mice (n=3 animals), T. brasiliensis (n=4 animals), and M. velifer (n=4 animals), presented as specific activity (fluorescence units/minute/100 μg protein/intensity of 20S signal from Western blot). Bars represent means ± se. F and P values represent 1-way ANOVA for data within either control or treated samples. B) Representative Western blot showing intensity of signal following incubation with primary antibody for 20S proteasome. Each lane represents 20 μg total protein from liver homogenates.