Figure 2.

PS containing Zn²⁺ expresses PS-direct in plasma. A) Endogenous thrombin potential assays were performed as described in Materials and Methods. NHP (dashed curve) or PSdP or mixtures of the 2 plasmas were compared to PSdP supplemented with 2 doses each of immunoaffinity-purified PS (IAP PS) that contained Zn²⁺ and conventionally MonoQ-purified PS (MQ PS, dotted curve) that was Zn²⁺ deficient. For clarity, only 1 dose of each PS and 1 mixture of NHP and PSdP are shown. B) Linear response of lag time in the ETP assay for various mixtures of NHP/PSdP or NHP/TFPIdP. Standard curves were used to estimate the PS-direct of Zn²⁺-containing or Zn²⁺-deficient PS added to PSdP or TFPIdP. C) Similar experiment as in A, but using TFPIdP in place of PSdP. All results were confirmed on separate dates.