Figure 6.

Binding of TFPI to PS. A) Binding of TFPI to Zn²⁺-containing PS (squares) or to Zn²⁺-deficient commercial PS (circles) immobilized on a plate at 2 μg/ml. Results were confirmed in a separate experiment. B) SPR binding experiments. See Materials and Methods for details. Peak binding of 2 different concentrations of TFPI to Zn²⁺-containing PS and 3 different concentrations of TFPI to commercial Zn²⁺-deficient PS. Data for the 2 PS were indistinguishable and were combined in one graph. Global fit of sensograms corresponding to these data is shown in Table 3.