Figure 7.

Antibodies against PS residues 621–635 (PSP14) preferentially recognize PS that contains Zn²⁺. Several PS preparations were coated to the wells of a microtiter plate at the variable concentrations indicated on the x axis. Immunoaffinity-purified antibodies against PSP14 were incubated in the wells, and bound antibodies were detected with biotinylated secondary antibodies as described in Materials and Methods. ERL PS, untreated Zn²⁺-deficient commercial PS; urea/Zn²⁺ ERL PS, same PS after treatment with Zn²⁺ in urea as in Fig. 3; IAP PS, Zn²⁺-containing immunoaffinity-purified PS.