TABLE 2.
Zn2+ incorporation and PS-direct of several PS preparations with low PS-direct
| PS preparation | Treatment | Zn2+(mol)
|
IC50(μM)
|
1/IC50
|
|||
|---|---|---|---|---|---|---|---|
| Pre | Post | Pre | Post | Pre | Post | ||
| Commercial PS-A | pH 2.7/Zn2+ | 0.1 | 0.6 | >2a | 0.40 | <0.5 | 2.5 |
| Commercial PS-B | pH 2.7/Zn2+ | <0.1 | 0.7 | >2 | 0.33 | <0.5 | 3.0 |
| MQ/Ca-purified PSb | pH 2.7/Zn2+ | 0.3 | 1.2 | ≥2 | 0.11 | ≤0.5 | 9.1 |
| Commercial PS-B | 6 M urea/Zn2+ | <0.1 | 1.4 | >2 | 0.39 | <0.5 | 2.6 |
| Commercial PS-B | 6 M chelexed urea | <0.1 | <0.1 | >2 | >2 | <0.5 | <0.5 |
a
Highest [PS] tested was 2 μM, which is 6-fold higher than the plasma [PS].
b
PS purified using 2 MonoQ (MQ) steps (see Table 1).