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. 2009 Jul;23(7):2215–2225. doi: 10.1096/fj.08-123737

Figure 3.

Figure 3.

Effect of hut-1 knockout and knockdown on the expression of Phsp-4::GFP and intestinal morphology. A) Knockout or knockdown of hut-1 induces Phsp-4::GFP reporter. DIC (top) and fluorescence (bottom) images of wild-type L4 (left), hut-1(tm1435) L4 (middle), and adult of hut-1(tm1435) rescued with hut-1 genomic DNA (right), which are expressing gfp under the control of hsp-4 promoter. B) Effect of control RNAi and hut-1 RNAi on Phsp-4::GFP analyzed by worm sorter COPAS Biosort. The fluorescence and time-of-flight (TOF) are expressed in arbitrary units. C, D) Intestinal phenotypes of various worms. C) DIC images of the intestines of wild-type, hut-1(tm1435), and rescued hut-1(tm1435) larvae. Intestines are outlined in dashed yellow lines. Scale bar = 50 μm. D) Intestinal granules visualized by DAPI filter in wild-type and hut-1(tm1435) larvae. Asterisks indicate position of the pharyngeal terminal bulb. Scale bars = 200 μm (A); 50 μm (C, D).