Figure 5.

PROK1 induces LIF expression in first trimester decidua. Treatment of first trimester decidua with 40 nM PROK1 induced LIF mRNA (A) and protein (B) expression that peaked at 6 and 12 h, respectively. Expression of LIF mRNA in first trimester decidua was measured after treatment with 40 nM PROK1 (lane 1) in the absence or presence of YM254890 (Gq inhibitor, lane 2), BAPTA-AM (calcium inhibitor, lane 3), PKC inhibitor (Ro-318220, lane 4), cSrc inhibitor (PP2, lane 5), EGFR inhibitor (AG1478, lane 6), or MEK inhibitor (PD98059, lane 7) (C). PROK1-induced LIF mRNA expression was significantly inhibited in the presence of these inhibitors. Bars represent means ± se of at least 3 independent experiments. *P < 0.05.