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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Mech Dev. 2007 Mar 12;124(6):409–415. doi: 10.1016/j.mod.2007.02.003

Fig. 1. Abnormal odontoblastic and ameloblastic differentiation in lower 1st molar teeth of E18.5 Tgfbr2fl/fl;Wnt1-Cre mice.

Fig. 1

In situ hybridization of Dspp, Col1a1 and Amelogenin using wild type (A,C,E) and Tgfbr2fl/fl;Wnt1-Cre (B,D,F) samples at E18.5. (A,B) Col1a1 is expressed in osteoblasts (arrowhead) in both wild type and Tgfbr2;wnt1-Cre mutant mice, but expression in Tgfbr2; Wnt1-Cre mice is dramatically reduced compared to wild type. (C,D) Dspp is expressed in odontoblasts and ameloblasts in wild type (arrow). In contrast, Dspp expression is not observed in Tgfbr2;wnt1-Cre mice. (E,F) Amelogenin expression is detected in ameloblasts in wild type mice (arrow), but is not detectable in Tgfbr2; wnt1-Cre mice. (G,H) In situ hybridization of Tgfbr2 using wild type 1st lower molar at E16.5 (G) and E18.5 (H). Tgfbr2 is expressed in dental mesenchymal cells at E16.5 (arrow) (G), and odontoblast cells at E18.5 (arrow) (H). Scale bars: 100 μm in A-F, 30 μm in G and F.